Buttercup brings the spice to the Powerpuff Girls. She and her sisters Blossom and Bubbles were all accidentally created by Professor Utonium on the Powerpuff Girls show. Out of all of them, this green crusader loves fighting the most, and has both an uncertain temper (she has been known to challenge Blossom's leadership) and the least tolerance for the softer, "girly" side of life. There has, however, been at least one occasion on which Buttercup has shown herself to be attracted to Ace D. Copular, the leader of the Gangreen Gang.
Before Fuse invaded Earth, Buttercup and her sisters were involved in a fight with Mojo Jojo in one of his newest mechs, as the girls were fighting, Buttercup began complaining about how played out Mojo's use of giant robots is becoming, much to the chagrin of Blossom and the agreement of Bubbles. While the girls distracted themselves Mojo takes the opportunity to attack Blossom with a charge blast, but before the blast hit, Buttercup pushed Blossom out of the way and took the blast head on. The impact knocked Buttercup unconscious and she fell into the ocean, but after her sisters defeated Mojo they went looking for her, but with no luck, and eventually she was declared missing. Her holographic image now stands in the Heroes Square Monument in her honor along with the other heroes whom have gone missing.